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Three strains which accumulated large quantities of L-hlutamic acid as the chief product of metabolism in the presence of glucose and inorganic ammonium salt under the aerobic condition sere newly isolated. These strains have the general characters as follows: Gram-positive, short rod-shaped, non-sporulating, non-motile, and facultative anaerobe. A change of cell shapes was not almost observed in their life cycles and neither a phenomenon of cell-elongation nor a pleomorphism was recognized in any cases. Together with rigid stability in Gram-stain, these characters as above indicated evidences that newly isolates would belong to genus Brevibacterium clearly differentiated from genus Corynebacterium. At the same time the newly isolates, in addition to nutritional requirement of biotin and thiamine, showed the distinctive character of requirement of special amino acid such as histidine or cysteine for their considerable growth. These physiological characters including nitrates reduction, reaction on milk, and slow formation of acid from sugar also were useful in confirming that these bacteria would be Brevibacterium ammoniagenes.
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